Valencia and Argentina defender Ezequiel Garay became the first La Liga player to announce a positive test earlier on Sunday.
Spain’s La Liga was suspended on Thursday after the Real Madrid squad went into quarantine.
Spain is the second worst-hit country in Europe after Italy and is set to declare a national lock down on Monday.
Ezequiel Garey wrote on his Instagram
It is clear I have started 2020 with bad luck
I am very well and must obey health authorities and stay isolated.
Garay, Mangala, Gaya and two Valencia staff members test positive for coronavirus.
A Valencia statement said
the affected players and staff were in their homes in good health and under isolation measures
We are confident that with solidarity, responsibility and good spirits we will beat this pandemic.